Product Info Summary
SKU: | A03586 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-MEKK3 MAP3K3 Antibody
SKU/Catalog Number
A03586
Size
100ul
Form
Liquid
Description
Boster Bio Anti-MEKK3 MAP3K3 Antibody catalog # A03586. Tested in WB,ICC/IF,IHC,IP,Flow Cytometry applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MEKK3 MAP3K3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03586)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human Dvl-3
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A03586 is reactive to MAP3K3 in Human, Mouse, Rat
Applications
A03586 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
70.898kDa
Background of MEKK3
The production of the second messenger molecules diacylglycerol. (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. It is a crucial enzyme in transmembrane signaling.
Singer, W. D. et al. (1997) Annu. Rev. Biochem. 66, 475-509.
Smrcka, A. V. et al. (1991) Science 251, 804-807.
Yue, C. et al. (2000) J. Biol. Chem. 275, 30220-30225.
Watanabe, D. et al. (2001) J. Biol. Chem. 276, 38595-38601.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1,000-1:2,000
ICC: 1:100-1:500
IHC: 1:50-1:200
FC: 1:50-1:100
Validation Images & Assay Conditions
Click image to see more details
Figure 2. Immunocytochemistry staining of MAP3K3 using Anti-MEKK3 MAP3K3 Antibody (A03586).
ICC staining MEKK3 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehydeClick image to see more details
Figure 1. Western blotting validation for Anti-MEKK3 MAP3K3 Antibody A03586
Western blot analysis of MEKK3 on different lysates using anti-MEKK3 antibody at 1/1Protein Target Info & Infographic
Gene/Protein Information For MAP3K3 (Source: Uniprot.org, NCBI)
Gene Name
MAP3K3
Full Name
Mitogen-activated protein kinase kinase kinase 3
Weight
70.898kDa
Superfamily
protein kinase superfamily
Alternative Names
EC 2.7.11.25; MAP/ERK kinase kinase 3; MAP3K3; MAPK/ERK kinase kinase 3; MAPKKK3; MAPKKK3MEKK 3; MEK kinase 3; MEKK3; mitogen-activated protein kinase kinase kinase 3 MAP3K3 MAPKKK3, MEKK3 mitogen-activated protein kinase kinase kinase 3 mitogen-activated protein kinase kinase kinase 3|MAP/ERK kinase kinase 3|MAPK/ERK kinase kinase 3|MEK kinase 3|MEKK 3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MAP3K3, check out the MAP3K3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MAP3K3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MEKK3 MAP3K3 Antibody (A03586)
Hello CJ!
No publications found for A03586
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Recommended Resources
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Customer Reviews
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